Characterization of Borrelia afzelii Isolated from Ixodes nipponensis and Apodemus agrarius in Chungju, Korea, by PCR‐RFLP Analyses of ospC Gene and rrf (5S)‐rrl(23S) Intergenic Spacer
Identifieur interne : 000B83 ( Main/Exploration ); précédent : 000B82; suivant : 000B84Characterization of Borrelia afzelii Isolated from Ixodes nipponensis and Apodemus agrarius in Chungju, Korea, by PCR‐RFLP Analyses of ospC Gene and rrf (5S)‐rrl(23S) Intergenic Spacer
Auteurs : Seung-Hyun Lee [Corée du Sud] ; Kwang-Don Jung [Corée du Sud] ; Jung-Hee Lee [Corée du Sud] ; Sang-Choin Kim [Corée du Sud] ; Jong-Hyun Kim [Corée du Sud] ; Won-Jong Jang [Corée du Sud] ; Kyung-Hee Park [Corée du Sud]Source :
- Microbiology and Immunology [ 0385-5600 ] ; 2002-10.
Abstract
Nine Borrelia afzelii strains, which had been isolated from two vectors, Ixodes nipponensis and Apodemus agrarius in Chungju, Korea, were characterized by PCR‐RFLP analyses of ospC genes and rrf (5S)‐rrl (23S) intergenic spacer. DraI restriction patterns of Chungju strains were identical to those of B. afzelii VS461. But MseI restriction patterns of rrf(5S)‐rrl (23S) intergenic spacer genes of KK2, KM4, KK5 differed from those of previously reported B. burgdorferi sensu lato strains. Nine Chungju strains were classified with four distinct ospC RFLP patterns, which differed from the eight ospC RFLP patterns (A‐1 to A‐8) of previously reported B. afzelii. Moreover, five additional restriction patterns were deduced from published ospC sequences of reference strains. These results suggest that Chungju strains are very heterogeneous.
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DOI: 10.1111/j.1348-0421.2002.tb02751.x
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DraI restriction patterns of Chungju strains were identical to those of B. afzelii VS461. But MseI restriction patterns of rrf(5S)‐rrl (23S) intergenic spacer genes of KK2, KM4, KK5 differed from those of previously reported B. burgdorferi sensu lato strains. Nine Chungju strains were classified with four distinct ospC RFLP patterns, which differed from the eight ospC RFLP patterns (A‐1 to A‐8) of previously reported B. afzelii. Moreover, five additional restriction patterns were deduced from published ospC sequences of reference strains. These results suggest that Chungju strains are very heterogeneous.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Lee, Seung Yun" sort="Lee, Seung Yun" uniqKey="Lee S" first="Seung-Hyun" last="Lee">Seung-Hyun Lee</name></noRegion><name sortKey="Jang, Won Ong" sort="Jang, Won Ong" uniqKey="Jang W" first="Won-Jong" last="Jang">Won-Jong Jang</name><name sortKey="Jung, Kwang On" sort="Jung, Kwang On" uniqKey="Jung K" first="Kwang-Don" last="Jung">Kwang-Don Jung</name><name sortKey="Kim, Jong Yun" sort="Kim, Jong Yun" uniqKey="Kim J" first="Jong-Hyun" last="Kim">Jong-Hyun Kim</name><name sortKey="Kim, Sang Hoin" sort="Kim, Sang Hoin" uniqKey="Kim S" first="Sang-Choin" last="Kim">Sang-Choin Kim</name><name sortKey="Lee, Jung Ee" sort="Lee, Jung Ee" uniqKey="Lee J" first="Jung-Hee" last="Lee">Jung-Hee Lee</name><name sortKey="Park, Kyung Ee" sort="Park, Kyung Ee" uniqKey="Park K" first="Kyung-Hee" last="Park">Kyung-Hee Park</name><name sortKey="Park, Kyung Ee" sort="Park, Kyung Ee" uniqKey="Park K" first="Kyung-Hee" last="Park">Kyung-Hee Park</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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